Our biostimulators are based on two principles of action:
- On the one hand, there is a direct effect on immunocompetent cells, as they are better supplied with oxygen, which in turn leads to an improved metabolic performance of these cells.
- The other effect is the influence of microbial mixed biocoenoses by special combinations of plant extracts. This leads to a deliberate shift in the composition of the flora and thus to a change in the metabolic rate of this flora.
1. Immunomodulation by Phase Transfer Catalysis
Patent no. DE 44 35 525 A1
The body of the animals works best under certain environmental conditions. In the body, biological processes regulate and compensate for the changing environmental conditions to ensure that body functions always run optimally. These biological processes involve a number of regulatory mechanisms that generally work perfectly. Sometimes, however, these control mechanisms fail or become less efficient, which in turn causes susceptibility to disease.
Many different performance depressions are causally related to dysfunctions of the body’s own control and regulation mechanisms and express themselves over the long term for example by immunodeficiencies, caregivers, poor fertility, increased mortality and atopic diatheses (eg allergies).
A non-functional immune system quickly has serious effects. Even trivial diseases can then be fatal. Maintaining a functioning immune system (a constitutional factor for “health”) is not just about having a stable psyche (happy people and animals are much less likely to catch colds) and less stress (but not stress), especially proper nutrition. An adequate supply of vitamins (especially C, B complex and E) and trace elements play a role as well as some polyuronic acids (eg from marine algae), especially in combination with special minerals.
This is especially true for brown algae alginic acid, which is rich in silyl esters in certain marine areas in a few months of the year. Together with divalent iron or magnesium, this substance has the property of transferring oxygen to cells, including immune-competent cells. These brown algae are harvested on the coasts of the cool seas (British Isles, Scandinavia, Brittany, Japan and China) for thousands of years for human and animal nutrition.
Experiments have shown that algins, in combination with certain cations, accelerate the transfer of oxygen in cell membranes – the so-called phase transfer catalysis (PTC). Thus, they support one of the most important tasks in the body: supplying the cells with oxygen. All body cells need oxygen, but most are undersupplied with it. As long as oxygen delivery across the blood is sufficient, the critical factor for supplying the cell is the solubility of oxygen in the lipid layer of the cell.
Polyuronic acids, consisting of block polymers of guluronans, form a cage formed by 4 molecules and 2 side chains. In the center of this cage is a divalent cation, which
- holds the two chains together
- is held by two covalent and two non-covalent bonds. These bonds rotate (Figure 1).
After attachment of the macromolecule to the cell wall, the functional sequence is always the same. The target cell or the target organ is dependent on the central cation used and / or the side chain of the polyuronic acid. In the case of the immunomodulator, the polyuronic acid (natural extract of brown algae) binds specifically to lymphocytes and macrophages, so immunocompetent cells.
The macromolecule attaches to the cell surface specifically of lymphocytes and acts there as a phase transfer catalyst. Better oxygenation causes faster cell division. More lymphocytes (B and T cells) mean a better immune response as more antibodies are produced and the cytotoxic response is increased.
A specific PTC serves to better supply macrophages with oxygen. One of the effects of this is the induction of an enzyme, NO synthase, which splits off arginine NO (nitric oxide). NO is a neurotransmitter in the gastrointestinal area, which also inhibits microorganisms (bacteria, viruses, plasmodia) in the immediate vicinity of the macrophage.
Numerous in vitro and in vivo experiments have shown that this processed alginic acid is capable of significantly increasing the immune system response in an unspecific manner. This applies to fish, birds, horses, dogs, pigs, cattle, mice, rats and guinea pigs as well as to humans.
A healthy immune system is not only necessary to cope with infections, but also to ensure high levels of performance. In most animals, the immune response deteriorates due to stress and more frequent vaccinations. The immune system can be improved by less stress and optimal feed composition. One can also reverse that: A good immune system makes less susceptible to stress.
2. Influence of Microbial Mixed Biocoenoses by Membrane Effectors
Patent no. DE 38 25 312 C2
With few exceptions, microorganisms naturally do not occur as a monoculture but as a mixed biotic community (microbial mixed biocoenosis). As an example, the cohabitation of rumen, soils or activated sludge in sewage treatment plants may be mentioned. The composition is by no means coincidental, but depends on the interactions between the organisms.
- Competition: Microorganisms partly compete for the same substrates. In this case, one species with certain survival advantages can displace another.
- Antibiosis: In the fight for survival directly other types can be attacked chemically, eg. For example, with antibiotics to defend against competitors. In this case one speaks of antibiosis.
- Substrate chains: If a species re-uses the end product of another species in the metabolism, then both depend on each other. Very often, the accumulation of a final product leads to an inhibition of the previous step (end product inhibition). Both species work as if on a conveyor belt and are thus able to carry out complex biochemical reactions. An example in the aerobic range is the oxidation chain ammonium / nitrite / nitrate by Nitrosomonas and Nitrobacter or the chain acetogenic bacteria / methanogenic bacteria in the methane fermentation (digestion). Also multi-chain systems are known. These are typical symbioses.
- Suppline: Another possibility of symbiosis is the coexistence of microorganisms, in which one species produces a substance that is essential for another species (a supplin) and can only live if the other species is present. Suppliners play the same role in microorganisms as essential vitamins or amino acids in humans and animals.
The interaction of competition, antibiosis, symbiosis and supplin addiction creates a network of relationships that leads to a very stable and at the same time flexible microbial society. This is maintained as long as the environmental conditions (temperature, pH, substrate supply) do not change drastically. The composition of such communities remains remarkably stable. If you try to introduce new microorganisms artificially, they are quickly displaced again. This elimination mechanism is structurally similar to the immune response of higher animals.
It is of interest to penetrate the intermicrobial communication system in order to influence composition and activity in a controlled way. One possibility for this is the use of membrane effectors. These are substances that act on the membranes of microorganisms to achieve a new stable mixed biocoenosis. The individual microorganisms react differently to these substances. Only then is it possible to change the mixed biocoenosis.
Since natural products serve as starting materials, it is necessary to analyze and standardize them.
In most cases, the use of membrane effectors in the normal, very small amounts used does not lead to changes, but to large quantities leads to labile new communities. In some cases, however, desirable stable new biocenoses are produced (with very small amounts too). It can be observed that the number of such stable states (in which the biocenosis represents a self-organizing system) is limited.